selective and differential media lab report conclusion

On the other hand, there are differential media which serve to differentiate organisms growing together. In the Mannitol Salt Agar Plate, there is Also E. coli would grow on Depending on the extent of breakdown, bacteria can give beta, alpha, and gamma outcomes, which show complete, partial, and absent hemolysis. It contains chemical compounds toxic to certain groups of bacteria and not others. WebConclusion. This culture medium can also be used to distinguish microorganisms that metabolize lactose from those that cannot utilize this sugar through a color indicator (Antony, Paul, & Silvester, & Abdulla, 2016). 2. 2. 5. Differential media: A differential medium contains additives that cause an observable color change in the medium when a particular chemical reaction occurs. The use of selective and differential agars in the isolation of Put in the stir bar and turn on the stir plate so that the surface is just disturbed. Today you will try to separate the bacteria in the mixture in order to obtain isolated colonies; next lab you will identify the two bacteria in your mixture and pick off single isolated colonies of each of the two bacteria in order to get a pure culture of each. StudyCorgi, 26 June 2021, studycorgi.com/bacteria-lab-enriched-selective-differential-media/. In the Eosin Methylene Blue agar, there is formation of a green metallic sheen. This means that it ferments glucose, decarbonizes lysine, ferments lactose, ferments, arabinose, and ferments sorbitol. In the two Tryptic Soy Agar plate, there was growth of colonies of Stir well to ensure a complete growth to support their growth. 4. WebA Selective Medium is a culture medium designed to allow the growth of some kinds of organisms, but not others. The products of chemical reactions after incubation give characteristic colony morphologies (Basu et al., 2015). Comparatively, the unknown bacterium exhibit similar properties to S. aureus because both did not show growth in EMB medium but exhibited growth in PEA medium. Thad Methylene Blue Agar plate and the other plate of Tryptic Soy Agar plate. Selective culture medium for the enumeration of Lactobacillus plantarum in the presence of 4) in order to see isolated colonies (see Fig. Selective growth media for eukaryotic cells commonly contain neomycin to select cells that have been successfully transfected with a plasmid carrying the neomycin resistance gene as a marker. Express your answer in terms of aaa and bbb. Eosin Y Dye (selective & differential) 51 Comments Please sign inor registerto post comments. Mannitol Salt Agar and its expected there would be a change in the medium color due to the Enriched Medium by Streak Plating. Growth = Not inhibited by Bile Salts - Gtam Negative It also shows how the Fieldwork and Research Methods in Anthropology, Agricultural Biotechnology and Its Pros and Cons. For instance, differentiating between inhibited growth and normal growth or beta-hemolysis and alpha-hemolysis was subjective. However, once it reaches other body tissues, it can lead to urinary tract infections, septicemia, wound sepsis, and enterococcal meningitis (Murray et al., 2015). You are free to use it to write your own assignment, however you must reference it properly. 9). NOT all Gram negative bacteria can ferment sucrose to produce acidic compounds Prepare medium of Eosin Methylene Blue, Mannitol Salt Agar and Tryptic Soy Agar However, problems such as contamination and subjective reading of culture plates might have influenced the outcome of results. Evolution of bacterial and fungal growth media. Eosin Y and Methylene Blue dyes inhibit the growth of Gram positive bacteria (only Gram negative bacteria will grow well), Differential Methylene Blue Dye (selective & differential) June 26, 2021. https://studycorgi.com/bacteria-lab-enriched-selective-differential-media/. WebDifferential Media. 8). This report is turned in after the class has had an opportunity to carefully look at the various plates which contain E. coli (EC), Proteus vulgaris (PV), Enterobacter; Question: Required LAB REPORT 3- Selected questions from exercise dealing with 3 selective / differential media as well as blood agar and tryptic soy agar. Report DMCA Overview WebExperimental rationale: Selective media can completely inhibit and/or slowly down the growth of certain species of bacteria. staphylococci which are halophilic bacteria. E. coli and Aureus respectively. The sterilize again and inoculate E. coli in Eosin Journal of Applied Some examples of selective media include: Differential media or indicator media distinguish one microorganism type from another growing on the same media. EMB and PEA media confirmed that the unknown bacterium was Gram-positive, whereas the blood agar eventually identified it as Streptococcus pyogenes because of its ability to give beta-hemolysis. On the other hand, strains that do not utilize lactose metabolize proteins, producing acids that increase the pH (Antony et al., 2016). Some of these strains P. aeruginosa and E. aerogenes are opportunistic pathogens that cause pulmonary, gastrointestinal, and urinary tract infections as well as sepsis, particularly in hospitalized patients (Murray et al., 2015). Thus, the findings confirmed that the unknown bacteria is not only Gram-positive but also beta-hemolytic one because it exhibited no growth in EMB, but growth in PEA medium with beta-hemolytic capacity. components. Bacterial growth need a medium of Objectives. WebSelective media = Media providing a means of isolating a particular species or type or microorganisms, while inhibiting the growth of others (e.g., EMB, MAC, T-7, MSA, etc.). Cross), Chemistry: The Central Science (Theodore E. Brown; H. Eugene H LeMay; Bruce E. Bursten; Catherine Murphy; Patrick Woodward), Brunner and Suddarth's Textbook of Medical-Surgical Nursing (Janice L. Hinkle; Kerry H. Cheever), Educational Research: Competencies for Analysis and Applications (Gay L. 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NOT all Gram negative bacteria can ferment sucrose to produce acidic compounds MacConkey agar, is selective and differential and is for Gram negative, against Gram positive, and also, differentiates colonies of lactose fermenting bacteria. Since EMB medium inhibits the growth of Gram-positive bacteria, it provided the basis for the identification of the unknown bacteria. Incubate the three plates upside down and stacked in the petri plate holder on the shelf of the 37C incubator corresponding to your lab section until the next lab period. c. Incubate the plates upside down in your petri plate holder at 37C until the next lab period. Shen, C., & Zhang, Y. MacConkey agar contains the dye crystal violet well as bile salts that inhibit the growth of most Gram-positive bacteria but do not affect the growth of most Gram-negatives. Bioinformation, 11(4), 182-184. the formation the observed growth and color. Single colonies will be visible on the 2nd or the 3rd streak. 3). The tube is placed on ice for a few minutes and if the media fails to solidify it is considered a positive test. Poor/No Growth - Organism inhibited by Bile salts and Crystal Violet - Gram Positive, EMB Agar is a selective AND differential media used to identify the presence of coliforms (Gram negative lactose fermentors) the properties of the two microbes used in the experiment. Staphylococcus aureus would grow on the Eosin Methylene Blue Agar since it its gram negative The Eosin and Methylene blue are dyes that have. Differential tests are a lot more precise than selective media. Typical colony morphology on MacConkey agar is as follows: Escherichia coli: colonies and confluent growth appear bright pink to red and surrounded by a pink precipitate (cloudiness) in the agar surrounding the growth (see Fig. Differential tests are sometimes the confirmation needed to determine what bacteria is being investigated. WebLab Report 2 - Selective And Differential Media December 2019 PDF Bookmark This document was uploaded by user and they confirmed that they have the permission to share it. The chemistry behind the test is that bacteria with exotoxins (hemolysins) have the capacity to break down red blood cells. resulted in te nature of the medium. MacConkey agar for Gram-negative bacteria. inoculated plates in an incubator at a temperature of 37 degrees Celsius for 48 hours. WebIt is crucial not to confuse the fact that testing is holistic with the much stronger conclusion that only wholes composed of theory, statistics, and experimental designs are jointly tested without ever being able to isolate anything specific shown by the evidence. some Gram negative bacteria can ferment lactose and/or sucrose Hektoen enteric agar (HE) which is selective for Gram-negative bacteria. The chemistry behind this test is that PEA increases the permeability of membranes of Gram-negative bacteria and allows an influx of various molecules into the bacteria, resulting in the inhibition of DNA replication. Antony, A., Paul, M. K., & Silvester, R., & Abdulla, M. H. (2016). "Bacteria Lab: Enriched, Selective, Differential Media." html5 version of animation for iPad showing how to streak an agar plate for isolation: 3 sector method. Key Differences Between Selective and Differential Media Selective media selectively isolate the particular strains of the organism, while differential medium In addition, it differentiates those organisms that grow based on whether they can carry out particular chemical reactions. 1. contains one or more specific compounds that can PREVENT the growth of certain bacterial species. Add 3.25 grams of the TSB powder to this flask and allow it to dissolve (will happen quickly). These will be your pure cultures for Lab 5 (Direct and Indirect stains). 3. black with a green metallic sheen (Shen & Zhang,2017). Research the oxidase test and describe how it is performed. WebCompared with diabetic patients with normal blood lipid, diabetic patients with dyslipidemia such as high triglycerides have a higher risk of clinical complications, and the disease is also more serious. Eosin Methylene Blue Agar being a selective medium favors the growth of gram negative skin, groin, armpit and other areas. Sucrose (differential) 2. Legal. WebMicrobiology Lab Report Use of Differential, Selective, and Enriched Media in Microbiology BIO 3150-700 Dr. Marilu Santos Introduction: The purpose of this experiment is to display the numerous special media used to isolate bacterial types, the differences between closely related groups of bacteria, the practice of sanitation and aseptic These findings may not be entirely correct because, we were not able to use certain cells in the enterotube due to lack of indole and other required, State College of Florida, Manatee-Sarasota. It further explains on the two types of sample organisms that the Aureus pathogenic staphylococci isolation. The yellow region is due to the fermentation of The Journal of Horticultural Science and Biotechnology, 19(2), 122-128. changes the red indicator to yellow color. It is, therefore, said to be selective for Gram-positive organisms, and would be useful in separating a mixture of Gram-positive and Gram-negative bacteria. 5. WebConclusion: The bacteria that we identified in our two enterotubes wereSerattia marsescenes, and Escheria coli.TheE.colitested positive for glucose, lysine, lactose, Enriched media are supplemented with nutrient sources and growth factors for culturing fastidious cells (Basu et al., 2015). pathogenic produces small yellow colonies within a yellow region. WebSome of the special-purpose media are as follows: 1. Possibly Shigella or Salmonella Scientific writing is written_x000D_ dif Enrichment media: An enrichment medium contains additives that enhance the growth of certain organisms. We have included the basic procedure for doing each biochemical test in the table below. Course Hero is not sponsored or endorsed by any college or university. streak plate method lab report conclusion January 7, 2022 A variety of techniques has been developed for the isolation of microorganism, mainly the bacteria, from the specimen or from the sample cultures. WebSelective media allow certain types of organisms to grow, and inhibit the growth of other organisms. Combination selective and differential media: A combination selective and differential medium permits the growth of one group of organisms while inhibiting the growth of another. Salmonella & Shigella CANNOT breakdown lactose, sucrose or salicin sugars an incubator at 37 degrees Celsius for 48 hours. Salmonella but NOT Shigella CAN reduce sulfur, Bile salts (selective) The Eosin Methylene Blue contains Staphylococcus aureus ferments mannitol, an acid byproduct is produced which subsequently An example of this selection would be using an increased salt concentration to only allow for the growth of halophilic bacteria. However, there are some strains that harmful that causes diarrhea id one drinks 3. coliform bacterium that is mostly found in the intestines and in the gut of some animals. streaking and make the 3rd streaking. While S. aureus exhibited gamma-hemolysis, the unknown bacteria depicted beta-hemolysis. Differential media. Orange/Yellow Growth = drop on pH = Large acidic = products made = fermentation of sugars = Not Salmonella or Shigella the growth of a broad variety of bacteria and other nutrients, it is non-selective and hence was WebThe aim of this experiment is to illustrate the uses of selective and differential media for the isolation as well as characterization of organisms of interest. in yoghurtA review. Find by direct integration the centroid of the area shown. The purpose of EMB as a selective medium is to allow the growth of Gram-negative bacteria but inhibits the growth of Gram-positive ones. WebA combination selective and differential medium permits the growth of one group of organisms while inhibiting the growth of another. There are possibilities that the streaking was done poorly, such as inoculating, too deep into the agar which would inhibit growth, or other mistakes when streaking such as not, using the "hood" cap correctly and allowing other particles to enter into the agar that was not part, Title: Enterotube II rapid identification system for Enterobacteriaceae, The purpose of this lab is to demonstrate the use of special agars in an Enterotube for, rapid identification of enteric bacteria. 4. Morphologically, enterococci and streptococci have spherical shapes. PINK & MUCOIDY bacterial colonies = SLIGHT change in media pH = SMALL amount of acidic products made = SLOW fermentation of lactose and differential media for the isolation as well as characterization of organisms of interest. differential mediums. EMB is useful in isolating and identifying Gram-negative enteric bacteria because it contains sucrose, lactose, and peptone complexed with eosin and methylene blue dyes. The gelatinase reaction may be slow or incomplete (Fig 7). Given that results of the experiment emanate from an accurate reading of plates, subjective reading was another problem encountered. The microbes have economic and medical relevance as disease-causing agents. WebHOW TO WRITE AN UNKNOWN LAB REPORT IN MICROBIOLOGY_x000D_ GENERAL_x000D_ Unknown reports in microbiology are written in scientific format. WebQuestion: Selective and differential media lab report This problem has been solved! 4 and Fig. These are used only for enteric bacteria which are found in the. From the results of the EMB test, Gram-positive strains (no growth) can be distinguished from Gram-negative cells. medium in a conical flask under sterile aseptic conditions. (2021, June 26). Comparative evaluation of EMB agar and hicrome E. coli agar for differentiation of green metallic sheen producing non E. coli and typical E. coli colonies from food and environmental samples. Selective media contain substances that will inhibit growth of organisms while allowing for only a specific type of organism to grow. A blood sample was then enriched medium used in this experiment. The growth of all microorganisms is dependent on favorable conditions of growth with Abstract This is because As a selective medium, PEA inhibits the growth of Gram-negative bacterium by increasing permeability of membranes and allowing molecules to enter into the cell and interfere with replication of DNA (Sirilun et al., 2017). CompareandContrast\text{\red{Compare and Contrast}}CompareandContrast Describe the difference between a reaction that often happens on its own and one that does not. Lab Report #11 - I earned an A in this lab class. and E. Coli bacteria, their properties and where they are situated (Talahmeh & Al-Razem, 2020). After 48hours, introduce the sample microbes Poor/No growth = Organism inhibited by Eosin and Methylene blue dye = Gram Positive Six plates for each media were prepared and inoculated with five known bacteria and the unknown bacteria using the streaking method. Flash animation showing how to streak an agar plate for isolation: 3 sector method. the commonly known varieties of staphylococcal bacteria. The acid being acidic in nature turens Students also viewed Evaluation of Antiseptics and Disinfectants Questions 1 Talahmeh, N., Abu Rumeileh, S., & Al Razem, F. (2020). The LibreTexts libraries arePowered by NICE CXone Expertand are supported by the Department of Education Open Textbook Pilot Project, the UC Davis Office of the Provost, the UC Davis Library, the California State University Affordable Learning Solutions Program, and Merlot. June 26, 2021. https://studycorgi.com/bacteria-lab-enriched-selective-differential-media/. WebSelective media = Media providing a means of isolating a particular species or type or microorganisms, while inhibiting the growth of others (e.g., EMB, MAC, T-7, MSA, etc.). Another method of separating bacteria is the pour plate method. Gancyclovir is an exception to the rule as it is used to specifically kill cells that carry its respective marker, the Herpes simplex virus thymidine kinase (HSV TK). Three types of growth media, namely, eosin methylene blue (EMB), phenyl ethyl alcohol agar (PEA), and blood agar, were used to differentiate and identify unknown bacterium. According to the EMB media above, which selects for growth of Journal of applied bacteriology, 81 (6), 663-668. Our provided with Amsterdam, Netherlands: Elsevier. In addition, it differentiates those organisms that grow based on whether they can carry out particular chemical reactions. MSA (mannitol salt agar) selective. Procedure: Selective and Differential Media 1. How are "superbugs" different from a wild type organism? BLUISH / GREEN bacterial colonies WITH BLACK PRECIPITATE 5. This is because it has got all the necessary nutrients and constituents that favor The plates were incubated at 370C for a day, an observation was made, and results tabulated. and inorganic salts, hardened by the addition of 1.5% agar. WebConclusion In the world with over 70% of internet users active on social networks, who spend at least one hour a day on average on those social networks, we have to conclude that social networks have become a sort of reality in which people communicate, interact, and obviously trust. Cool the loop then do the 2nd This will guide your streaking. depending on original sample density. One plate of each of the following media: Trypticase Soy agar, Columbia CNA agar, and MacConkey agar. WebThe media is checked over a period of about a week after inoculation and incubation at room temperature, for gelatinase activity. Web1st steps in biochemical determination of an organism. 2. Isolation of bacteria is accomplished by growing ("culturing") them on the surface of solid nutrient media. and for Tryptic Soy Agar pour into two separate mediums. contaminated water or stale food. After completing this lab, the student will be able to complete the following objectives: 1. The purpose of PEA is a selective medium because it prevents the growth of Gram-negative bacteria but allows the growth of Gram-positive bacteria. Methicillin-resistant staphylococcal skin infections by S. aureus are prevalent in diabetic, elderly, and immunocompromised populations. However there was no indole used and some of the agars, were not used in our observations. result of the dyes metachromatic properties and also due to the robust acid end product test of Blood agar is another form of media that the experiment used in the identification of the unknown bacterium. This plate is called an isolation plate. The pink region is due to the effect of other Web. formation of two zones, one is yellow and the other pink. Bacteria Lab: Enriched, Selective, Differential Media. Streak the same mixture for isolation (see Fig. The other varieties of bacteria are inhibited. Basu, S., Bose, C., Ojha, N., Das, N., Das, J., Pal, M., & Khurana, S. (2015). WebSelective and differential media are media based on either complex or defined media supplemented with growth-promoting or growth-inhibiting additives.

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